Reversible IC 50 and time-dependent K I and k inact values were used for predictions of the potential clinical impact of the inhibition of CYP2C8 or CYP3A4
The objectives of this study were to characterize and compare the reversible inhibition and time-dependent inactivation of cytochromes P450 3A4 and
In vivo and in vitro
MA and MD were reversible inhibitors of CYP3A with competitive Ki values of 2
CCB is generally considered a preferred antihypertensive agent for KTRs 18
Diltiazem (DTZ) N-demethylation
Recent guidance from the US Food and Drug Administration (USFDA) has advocated testing of time-dependent inhibition of cytochrome P450 (CYP), which can be addressed by performing IC (50) shift as well as K (I)/k (inact) determinations
In this study, we evaluated the contributions of inactivation and reversible inhibition of CYP3A
Simulated assay conditions [S] = 10 μM (K M), [E] = 100 pM, k cat = 1 μM s − 1
AU - Burt, Howard J
However, measuring TDI of CYP3A in human liver microsomes (HLMs) frequently yields overestimations of clinical DDIs and thus can lead
The clinical impact of drug-drug interactions based on time-dependent inhibition of cytochrome P450 (CYP) 3A4 has often been overpredicted, likely due to use of improper inhibitor concentration FIG
Time-Dependent Inactivation of CYP3A4 and CYP3A5 Activity
30 Berry LM Zhao Z An examination of IC50 and IC50-shift experiments in assessing time-dependent inhibition of CYP3A4
After 7 days, adrenal insufficiency and resistant hypertension improved and were accompanied by a significant reduction in the urinary 6β-OHF:F ratio
Ultimately, this simplified system was used to reveal insights into CYP3A4 biochemical behavior
In this study, we use two cysteine- Here, we investigated and confirmed that ERD inactivated both CYP3A isoforms in a time-, concentration-, and NADPH-dependent manner with K I, k inact, and partition ratio of 4
(iv) The structures of many of the drugs that show time-dependent inhibition are Azamulin is used as a selective inhibitor for 3A4 and 3A5 to define their roles in metabolism of new chemical entities during drug development
The consequences of TDI can be termination of drug development, drug withdrawal or serious restrictions of use