Chloramphenicol plates recipe

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  • How to make a 25-50 mg/ml Chloramphenicol Stock Solution
  • TIP: reduce the power to 50 W and melt for 5-6 min
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  • Swirl to mix the antibiotic uniformly in the media 7
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  • Begin by sterilizing the petri dishes
  • Heat to boiling to
  • Chloramphenicol Stock Solution Preparation and Recipe
  • Adjust to pH 5
  • ddH 2 O
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  • Add 10 g of Penicillin G to the solution
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  • After Using a sterile pipette tip or toothpick, select a single colony from your LB agar plate

    The use of Shrink-Seals (REF R522600) or gas-permeable tape to secure the plates is recommended to prevent exogenous contamination, avoid moisture loss, and protect personnel from exposure to pathogenic molds

    This Thermo Scientific Chemicals brand product was originally part of LB-kanamycin agar plates

    Plates will keep refrigerated for 4-6 weeks

    0 g agar-agar 1 L Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x

    How to make a 25-50 mg/ml Chloramphenicol Stock Solution

    Chloramphenicol – add 1ml chloramphenicol stock (at 25mg/ml) per liter of agar to obtain a final concentration of 100ug/ml

    In most cases you will add 1 ml of stock antibiotic per liter of solution

    Add about 800mL of dH O and dissolve the big chunks and stir with a magnetic bar until it's

    Pour hot/ warm (melted) media at 20ml per petri dish [Note: stack plates on top of each other when preparing several plates to preven plates from sweating] PREPARATION OF LB-AMP PLATE (500ml) Add 12

    Thaw and mix (stir) 100 mM stock solutions of dGTP, dATP, dTTP, dCTP (NEB; in freezer) Master Mix: 60 μl sterile dH 2 O

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    Then, filter sterilize solutions by pushing them through a 50 mL syringe fitted with a 0

    In general, use carbenicillin for ampicillin selection Plate 50 µL of transformed E

    Ampicillin (Amp/A): *Working concentration 100 ug/mL freezer stock 100 mg/mL (in water) Add 1 mL of 100 mg/mL ampicillin (dissolved in water) into 1

    Note: If desired, 20 units of Penicillin and 40 µg Streptomycin per ml of the medium may be added to make the medium selective for fungi

    North American Regional Complaint Center 1-844-8BD-LIFE (1-844-823-5433)

    For 500 milliliters (ml) of agar mixture, you can fill 25 average 1

    Cool to 55°C

    Prepare the desired volume of stock solution by dissolving chloramphenicol (Sigma-Aldrich C1919) at 10 mg/mL (or 20 mg/mL) in absolute ethanol

    Add the appropriate amount of desired antibiotic to the solution (500µL if you are using a 1,000x antibiotic stock) and swirl to mix

    For use, add 5 mL of the antibiotic mix to 1 L of Chloramphenicol (methanol) 10 20 10 40 80 0

    2

    I reduced the chloramphenicol concentration to an intermediate concentration of 68 ug/ml (about 2

    To process the specimen, stream the specimen being studied on the medium using a sterile inoculating loop

    Add Water and Stir until clumps are gone

    You may need to tilt the plate slightly to get the agar to Principle of Sabouraud Dextrose Agar (SDA) Sabouraud Dextrose Agar contains digests of animal tissues (peptones) which provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi and yeasts

    5 ug/mL final concentration) 2

    Pour ~20mL per 10cm

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    Add 40 μL of the Thermo Scientific X-Gal Solution (20 mg/mL You'll get a detailed solution from a subject matter expert that helps you learn core concepts

    10 μl dGTP

    Sabouraud dextrose agar is a selective medium for it is used to isolate yeast and fungi and at the same time permit the growth of filamentous bacteria like Nocardia

    Potato Dextrose Agar (PDA) P otato Dextrose Agar (PDA) is a common fungal growth media made from an infusion of potato and dextrose

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  • Chloramphenicol plates recipe
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